| 1 | –Brother Porter & Brother Cook | I Know My Jesus Won't Deny MeChorus – The PorterettesVocals – Brother Cook, Brother Porter | 2:41 |
| 2 | –W. Lee O'Daniel & Lightcrust Doughboys | Doughboys Theme Song #1 | 0:41 |
| 3 | –The Hokum Boys | Gin Mill BluesPiano, Vocals – Jimmy BlytheVocals – Banjo Ikey Robinson | 3:27 |
| 4 | –Breaux Freres | La Valse Des Yeux BleusAccordion, Vocals – Amédée BreauxGuitar, Vocals – Clifford BreauxViolin, Vocals – Ophy Breaux | 3:11 |
| 5 | –Will Batts | Highway #61 BluesGuitar – Jack Kelly Guitar, Voice – Dan SingVocals – Will Batts | 2:42 |
| 6 | –Washington Phillips | Paul & Silas In JailVocals, Zither [Dolceola] – Washington Phillips | 2:42 |
| 7 | –Blue Ridge Ramblers | D Blues | 2:48 |
| 8 | –Kid Prince Moore | South Bound BluesGuitar, Vocals – Kid Prince Moore | 3:01 |
| 9 | –Tom Darby & Jimmie Tarlton | Lonesome Frisco LineGuitar, Vocals – Tom DarbySteel Guitar, Vocals – Jimmie Tarlton | 3:17 |
| 10 | –Joe Williams | Baby, Please Don't GoBass – Ransom KnowlingGuitar, Vocals – Joe WilliamsHarmonica – Sonny Boy Williamson | 2:57 |
| 11 | –Bob Clifford | Onion Eating MamaGuitar, Vocals – Cliff Carlisle | 2:54 |
| 12 | –The Anglin Twins | Just Inside The Pearly GatesBass, Vocals – Jim AnglinGuitar, Vocals – Jack Anglin | 2:25 |
| 13 | –Callahan Bros. | Brown's Ferry Blues #2Guitar, Vocals – Homer Callahan, Walter Callahan | 3:06 |
| 14 | –The Alley Boys Of Abbeville | Pourquoi Tu M'Aime PasDrums – Maxie ToutchGuitar – Murphy Guidry, Sidney GuidryResonator Guitar [National] – Lourse ToutchViolin, Vocals – Frank Mailhes | 3:46 |
| 15 | –Bailes Bros. | You Can't Go Halfway (And Get In)Vocals – John Bailes, Walter Bailes | 2:46 |
| 16 | –Hersal Thomas | Suitcase BluesPiano – Hersal Thomas | 2:36 |
| 17 | –Bill Landford & The Landfordaires | Run On For A Long TimeLeader, Guitar – Bill Landford | 2:34 |
| 18 | –Homer Harris | Tomorrow May Be Too LateBass – Ransom KnowlingGuitar – Muddy WatersPiano – James Clark Vocals – Homer Harris | 3:05 |
| 19 | –The Humbard Family | I'll Fly AwayPerformer – Clement Humbard, Leona Humbard, Rex Humbard, Ruth Humbard | 2:49 |
| 20 | –Big Bill Broonzy | C & A BluesGuitar – Louis Lasky Guitar, Vocals – Big Bill Broonzy | 2:58 |
| 21 | –Blues Birdhead | Mean Low BluesHarmonica – Blues Birdhead | 3:13 |
| 22 | –Silver Leaf Quartet | Oh! Glory GloryBaritone Vocals – William BoushBass Vocals – Ellis McPhersonTenor Vocals – Melvin Smith Vocals [Falsetto] – William Thatch | 3:13 |
| 23 | –Pelican Wildcats | Walkin' Georgia Rose | 2:56 |
| 24 | –Pink Anderson & Simmie Dooley | C.C. & O. BluesGuitar, Vocals – Pink Anderson, Simmie Dooley | 3:05 |
| 25 | –Daniels-Deason Sacred Harp Singers | Hallelujah | 2:58 |
| 26 | –Charlie Patton | Jersey Bull BluesGuitar, Vocals – Charlie Patton | 3:04 |
| 27 | –Walter Roland | Every Morning BluesPiano, Vocals – Walter Roland | 2:44 |
| 28 | –W.T. Narmour & S.W. Smith | Sweet Milk And Peaches (Breakdown)Fiddle – W.T. NarmourGuitar – S.W. Smith | 3:11 |
| 29 | –Eldon Baker And His Brown County Revelers | One Eyed SamVocals – Harry Adams | 2:51 |
| 30 | –Frank Hutchison | The Last Scene Of The TitanicGuitar, Vocals – Frank Hutchison | 3:30 |
| 31 | –Barbecue Bob | Blind Pig BluesGuitar, Vocals – Barbecue Bob | 3:02 |
| 32 | –W. Lee O'Daniel And His Hillbilly Boys | Ida (Sweet As Apple Cider) | 3:09 |
| 33 | –James "Beal Street" Clark | Who But YouClarinet – J.T. BrownPiano, Vocals – James Clark | 2:38 |
| 34 | –Reverend Benny Campbell | Have Mercy On Me | 2:32 |
| 35 | –Peetie Wheatstraw | Police Station BluesGuitar, Vocals – Peetie Wheatstraw | 3:03 |
| 36 | –Dallas String Band With Coley Jones | Hokum BluesMandolin – Coley Jones | 3:25 |
| 37 | –Albert Ammons | Shout For JoyPiano – Albert Ammons | 2:25 |
| 38 | –Joe Falcon Accompanied By Clemo Breaux & Ophy Breaux | OssonAccordion, Vocals – Joe FalconGuitar – Clemo BreauxViolin – Ophy Breaux | 2:56 |
| 39 | –Poor Boy Burke | Old Vets BluesVocals – Poor Boy Burke | 2:53 |
| 40 | –Elizabeth Johnson | Empty Bed Blues Part 2Cornet – King OliverPiano – Clarence WilliamsVocals – Elizabeth Johnson | 3:22 |
| 41 | –The Nite Owls | Memphis BluesLeader – Jack TrueVocals – Luke Owens | 2:21 |
| 42 | –Tony Hollins | Cross Cut Saw BluesGuitar, Vocals – Tony Hollins | 2:48 |
| 43 | –Molly O'Day & The Cumberland Mountain Folks | Heaven's RadioGuitar, Vocals – Lynn Davis , Molly O'Day | 2:49 |
| 44 | –Gladys Bentley | Worried BluesPiano, Vocals – Gladys Bentley | 2:45 |
| 45 | –Dora Carr | Cow-Cow BluesPiano – Cow-Cow DavenportVocals – Dora Carr | 2:50 |
| 46 | –Peter Cleighton | Black Snake BluesGuitar – Robert Jr. LockwoodPiano – Blind John DavisVocals – Peter Cleighton | 2:51 |
| 47 | –Hershel Brown And His Happy Five | Liberty | 2:54 |
| 48 | –Ernest V. Stoneman's Trio | UntitledGuitar – Ernest V. Stoneman | 3:01 |
| 49 | –Elizabeth Johnson | Empty Bed Blues Part 1Cornet – King OliverPiano – Clarence WilliamsVocals – Elizabeth Johnson | 3:02 |
| 50 | –Mamie Smith | My Sportin' ManAccompanied By – Billy Fowler & His OrchestraVocals – Mamie Smith | 2:58 |
| 51 | –Freeny's Barn Dance Band | Don't You Remember The TimeFiddle – Hendrix Freeny, Leslie FreenyGuitar – Fonzie CannonMandolin – Cleveland Freeny | 3:16 |
| 52 | –Papa Too Sweet & Harry Jones | (Honey) It's Tight Like ThatGuitar – Tampa RedPiano, Guitar – Tom DorseyVocals – Harry Jones , Papa Too Sweet | 2:36 |
| 53 | –Willie "Boodle It" Right | Two By Four BluesPiano, Vocals – Willie Right | 2:56 |
| 54 | –Rosetta Howard | Plow Hand BluesAlto Saxophone – Sax MallardBass – Ransom KnowlingDrums – Judge RileyGuitar – Big Bill BroonzyPiano – Bob CallTenor Saxophone – Bill CasimirTrumpet – Johnny MortonVocals – Rosetta Howard | 2:46 |
| 55 | –Bessie Jackson | Skin Game BluesPiano – Walter RolandVocals – Lucille Bogan | 2:57 |
| 56 | –Bob And Randall Atcher | Papa's Going Crazy, Mama's Going MadVocals – Bob Atcher, Randall Atcher | 2:38 |
| 57 | –George Curry | My Last Five DollarsGuitar – Hobson "Hop" JohnsonVocals – George Curry | 2:36 |
| 58 | –Bill Monroe & His Bluegrass Boys | Goodbye Old PalAccordion – Wilene "Sally Anne" ForresterBanjo – David "Stringbean" AkemanBass – Bill WestbrookFiddle – Chubby WiseGuitar – Tex WillisMandolin, Vocals – Bill Monroe | 2:23 |
| 59 | –Vance's Tennessee Breakdowners | Washington County Fox Chase | 2:57 |
| 60 | –W. Lee O'Daniel & Lightcrust Doughboys | Doughboys Theme Song #2 | 0:29 |
| 61 | –Black Cats And The Kitten | Step It Up And GoVocals – Valda Buggs | 2:48 |
| 62 | –South Georgia Highballers | Blue Grass TwistGuitar – Vander Everidge | 2:48 |
| 63 | –Clarence Green | Johnson City BluesGuitar – Clarence Green | 2:57 |
| 64 | –Roy Harvey & Leonard Copeland | Back To The Blue RidgeGuitar – Leonard Copeland, Roy Harvey | 2:55 |
| 65 | –Big Maceo Merriweather | Maceo SpecialDrums – Tyrell DixonPiano – Big Maceo Merriweather | 2:42 |
| 66 | –Jack Kelly | Flower BluesGuitar – Little Son JoeGuitar, Vocals – Jack Kelly Violin – Will Batts | 2:30 |
| 67 | –Buster Carter & Preston Young | Darn Good GirlBanjo – Buster CarterFiddle – Posey RorerGuitar – Preston Young | 2:51 |
| 68 | –The Two Charlies | Tired Feelin' BluesGuitar, Vocals – Charlie Jordan, Charlie Manson | 3:03 |
| 69 | –The Light Crust Doughboys With J.B. Brinkley | It's Funny What Love Will Make You DoVocals – J.B. Brinkley | 2:47 |
| 70 | –Reverend Johnny Blakey Assisted By The Sanctified Singers | Warming By The Devil's FireGuitar – Lonnie McIntorshVocals – Bessie JohnsonVoice – Reverend Johnny Blakey | 3:25 |
| 71 | –Hank Penny And His Radio Cowboys | Army BluesAccordion – Kelland ClarkBass – Carl Stewart Guitar, Vocals – Hank PennyHarmonica – Eddie Smith Steel Guitar – Jimmy Colvard | 2:36 |
| 72 | –McKinley Morganfield | Burying Ground BluesBass – Ransom KnowlingGuitar – Leroy FosterGuitar, Vocals – Muddy WatersPiano – James Clark | 2:30 |
| 73 | –Sweet Violet Boys | You Got To See Mama Every Night (Or You Can't See Mama At All)Accordion – Augie KleinBass – Jack Taylor Guitar – Allan Crockett, Earl BackusMandolin – Chick HurtViolin – Salty HolmesVocals – Gale Ryan | 2:49 |
| 74 | –Leroy Carr With Scrapper Blackwell | Good Woman BluesGuitar, Voice – Josh WhitePiano, Vocals – Leroy CarrVoice – Scrapper Blackwell | 2:57 |
| 75 | –Tindley Quaker City Gospel Singers | Hallelujah SideBaritone Vocals – Harvey Mason , Thomas Brown Bass Vocals – Haywood PurnellTenor Vocals – Albert T. Tindley, D.C. Wright | 3:16 |
| 76 | –A'nt Idy Harper And The Coon Creek Girls | Poor Naomi WiseBass – Evelyn "Daisy" LedfordFiddle – Lily May LedfordGuitar – Rosie LedfordMandolin – Esther "Violet" KoehlerVocals – A'nt Idy Harper | 3:36 |
| 77 | –Charlie Bowman And His Brothers | Moonshiner And His MoneyBanjo – Walter BowmanFiddle, Voice – Charlie BowmanGuitar, Voice – Frank Wilson | 3:10 |
| 78 | –Lonnie Johnson | I Have To Do My TimePiano, Vocals – Lonnie Johnson | 3:09 |
| 79 | –Blind Willie | Bell Street Lightnin'Guitar – Curley WeaverGuitar, Vocals – Blind Willie McTell | 2:52 |
| 80 | –Reverend J.M. Gates | Death's Black Train Is Coming | 3:11 |
| 81 | –The Rhythm Wreckers | Blue Yodel #2 (My Lovin' Gal Lucille)Vocals – Whitey McPherson | 2:46 |
| 82 | –Little Son Joe | Black Rat SwingBass – Alfred ElkinsGuitar – Memphis MinnieGuitar, Vocals – Little Son Joe | 2:52 |
| 83 | –Whistlin' Alex Moore | They May Not Be My ToesPiano, Vocals – Whistlin' Alex Moore | 3:02 |
| 84 | –Little Buddy Doyle | Slick Capers BluesGuitar, Vocals – Little Buddy Doyle | 2:35 |
| 85 | –Charlie Poole With The North Carolina Ramblers | Whitehouse BluesFiddle – Posey RorerGuitar – Roy HarveyVocals, Banjo – Charlie Poole | 3:25 |
| 86 | –Whistler And His Jug Band | Low Down BluesJug – Rudolph ThompsonMandolin – Willie BlackViolin – Jess FergusonWhistle [Nose Whistle], Guitar, Vocals – Whistler | 2:56 |
| 87 | –The Okeh Atlanta Sacred Harp Singers | Ortonville | 3:11 |
| 88 | –Lonnie Johnson & Violet Green | You Had Too MuchGuitar, Vocals – Lonnie Johnson Piano – Alex HillVocals – Clara Smith | 3:19 |
| 89 | –Sherman Tedder | UntitledGuitar – Sherman Tedder | 2:57 |
| 90 | –Fiddlin' John Carson | I'm Going To Take The Train To CharlotteFiddle, Vocals – Fiddlin' John Carson | 3:05 |
| 91 | –Gene Autry | Dixie CannonballGuitar – Merle TravisVocals – Gene Autry | 2:40 |
| 92 | –Slim Doucet | Dear Black Eyes (Chere Yeux Noirs)Accordion – Slim DoucetGuitar – Chester Hawkins | 3:00 |
| 93 | –Bill McKinley | Poor Boy BluesGuitar – Big Bill BroonzyHarmonica, Vocals – Jazz GillumWashboard – Washboard Sam | 2:54 |
| 94 | –Mississippi Sheiks | The Jazz FiddlerGuitar, Vocals [Possibly] – Walter VincsonViolin, Vocals [Possibly] – Lonnie Chatman | 3:13 |
| 95 | –Pinewood Tom | Sissy ManGuitar, Vocals – Josh WhitePiano – Walter Roland | 2:49 |
| 96 | –Bo Carter | West Jackson BluesGuitar, Vocals – Bo Carter | 3:17 |
| 97 | –Mississippi John Hurt | Big Leg BluesGuitar, Vocals – Mississippi John Hurt | 2:51 |
| 98 | –Frank Edwards | We Got To Get TogetherHarmonica, Vocals – Frank EdwardsWashboard – Washboard Sam | 2:36 |
| 99 | –Sister Myrtle Fields Acc. By The Austin McCoy Trio | I'm ToilingOrgan – Austin McCoyVocals – Sister Myrtle Fields | 2:43 |
| 100 | –Gid Tanner & The Skillet Lickers With Riley Puckett & Clayton McMichen | Soldier's JoyBanjo – Fate NorrisFiddle – Clayton McMichen, Gid TannerGuitar – Riley Puckett | 2:54 |
| 101 | –Aiken County String Band | High Sheriff | 2:53 |
| 102 | –Deep South Boys | Until I Found The LordPiano – Thomas Griffin Vocals – Claude Robinson, David Mells, Harold Matthews, Marshall Derricotte | 2:25 |
| 103 | –Adolf Hofner And His San Antonians | Cotton-Eyed Joe | 2:29 |
| 104 | –Austin & Lee Allen | Chattanooga BluesGuitar, Kazoo – Lee Allen Tenor Banjo, Vocals – Austin Allen | 3:04 |
| 105 | –Roosevelt Sykes | Roosevelt BluesPiano, Vocals – Roosevelt Sykes | 2:47 |
| 106 | –Bumble Bee Slim | Hard Rocks In My BedPiano – Black BobVocals – Bumble Bee Slim | 2:40 |
| 107 | –Memphis Seven | Grunt Meat BluesAlto Saxophone – Porter KilbertBass – Truck ParhamDrums – Jump JacksonGuitar – Luther JonesPiano – Sugarman PenigarTenor Saxophone, Vocals – Cozy EgglestonTrumpet – Johnny Morton | 2:47 |